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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Rab10 Antibody #4262

Filter:
  • WB
Western Blotting Image 1: Rab10 Antibody
Western blot analysis of extracts from various cell lines using Rab10 Antibody.

To Purchase # 4262

Cat. # Size Qty. Price
4262S 100 µl
$306

Supporting Data

REACTIVITY H M Mk
SENSITIVITY Endogenous
MW (kDa) 23
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Rab10 Antibody detects endogenous levels of total Rab10 protein.

Species Reactivity:

Human, Mouse, Monkey

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp114 of human Rab10 protein. Antibodies are purified by peptide affinity chromatography.

Background

Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Mss4, myosin V (Va, Vb and Vc) and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosomes and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for insulin-stimulated GLUT4 translocation in adipocytes (7,8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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