Render Target: STATIC
Render Timestamp: 2024-11-25T11:53:27.999Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:27:57.636
Product last modified at: 2024-05-30T07:06:00.492Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Rab27B Antibody #44813

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 27
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rab27B Antibody recognizes endogenous levels of total Rab27B protein. Overexpression analysis confirms that this antibody does not detect Rab27A.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab27B protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Rab27 proteins are members of the Ras superfamily of small Rab GTPases associated with the process of exocytosis (1-2), and in particular the exosome secretion pathway (3-5). There are two Rab27 isoforms, Rab27A and Rab27B, which are encoded by separate genes and which exhibit distinct subcellular localization and functions. Rab27A colocalizes to CD63+ vesicles, whereas Rab27B is observed in perinuclear vesicles. Targeted knockdown studies suggest that the isoforms control distinct steps of the exosome secretion pathway (6). Rab27A participates in docking and membrane fusion from multivesicular endosomes (MVEs) to exosomes, whereas Rab27B mediates the transfer of membrane vesicles from the trans-Golgi network to MVEs. It has been suggested that Rab27A and Rab27B may have importance in exosome secretion by cancer cells (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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