Render Target: STATIC
Render Timestamp: 2024-12-11T11:05:55.313Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-01 15:25:13.216
Product last modified at: 2024-11-14T14:00:17.849Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Rab35 Antibody #9690

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 24
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Rab35 Antibody detects endogeneous levels of total Rab35 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp124 of human Rab35 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Rab35 belongs to the small GTPase superfamily. By interacting with its specific GEF or GAP, Rab35 regulates cargo-specific endocytosis at the vesicle recycling step or mediates exocytosis at the exosome docking/tethering step (1-4). During cytokinesis, Rab35 interacts with OCRL phosphatase and is essential for maintaining intercellular bridge stability and abscission by controlling the concentration of phosphatidylinositol 4,5-bisphosphate (PIP2) and SEPT2 localization at the intercellular bridge (5,6). Rab35 also plays a role in actin assembly and the recruitment of Cdc42 and Rac1 to the site of filopodium by its direct interaction with actin-bundling protein fascin and actin-binding protein connecdenn 3 (7-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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