Rab6 (D37C7) Rabbit mAb #9625
- WB
- IP
- IF
Supporting Data
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 24 |
Source/Isotype | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
Protocol
Specificity / Sensitivity
Rab6 (D37C7) Rabbit mAb recognizes endogenous levels of total Rab6 protein.
Species Reactivity:
Human, Mouse, Rat
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Rab6 protein.
Background
Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A', and Rab6B) are structurally similar but likely exhibit non-overlapping functions (2,3). Rab6 localized to the Golgi (4) regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6-mediated transport requires a functionally intact microtubule system (9,10). Rab6 also regulates cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12).
Limited Uses
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