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Render Timestamp: 2024-12-13T10:56:45.945Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:55:49.365
Product last modified at: 2024-12-05T14:00:14.352Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. RACK1 (D59D5) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RACK1 (D59D5) Rabbit mAb #5432

Filter:
  • WB
  • IP
  • IHC
  • eCLIP

    Supporting Data

    REACTIVITY H M R Mk Dm
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • eCLIP-eCLIP 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:400 - 1:1600
    eCLIP 1:200
    For more information about the RBP-eCLIP service please visit Eclipsebio.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #37124.

    Protocol

    Specificity / Sensitivity

    RACK1 (D59D5) Rabbit mAb recognizes endogenous levels of total RACK1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RACK1 protein.

    Background

    The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5).

    Database Links

    UniProt ID: P63244


    Entrez-Gene Id: 10399


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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