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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RACK1 (D59D5) Rabbit mAb #5432

Filter:
  • WB
  • IP
  • IHC
  • eCLIP
Western Blotting Image 1: RACK1 (D59D5) Rabbit mAb
Western blot analysis of extracts from various cell types using RACK1 (D59D5) Rabbit mAb.

To Purchase # 5432

Cat. # Size Qty. Price Ships
5432T 20 µl
$153 Jan 22
5432S 100 µl
$339 Jan 22

Supporting Data

REACTIVITY H M R Mk Dm
SENSITIVITY Endogenous
MW (kDa) 32
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • eCLIP-eCLIP 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Dm-D. melanogaster 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:400 - 1:1600
eCLIP 1:200
For more information about the RBP-eCLIP service please visit Eclipsebio.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #37124.

Protocol

Specificity / Sensitivity

RACK1 (D59D5) Rabbit mAb recognizes endogenous levels of total RACK1 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey, D. melanogaster

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RACK1 protein.

Background

The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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