Rad23A Antibody #14636
Inquiry Info. # 14636
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Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 52 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The UV excision repair protein Rad23A is a multi-domain scaffold protein that plays an important role in ubiquitin-dependent proteasomal degradation. Rad23A protein contains an amino-terminal ubiquitin-like (UBL) domain and two ubiquitin binding domains, UBA1 and UBA2 that flank an XPC binding domain (2). Rad23A is thought to form a closed conformation that is dictated by an intramolecular interaction between the UBL and UBA domains. Binding of the Rad23A UBL domain to the S5a/PSMD4 subunit of the proteasome lid disrupts the intramolecular UBL-UBA association within Rad23A and promotes its association with the proteasome (3). Research studies show that Rad23A can be recruited to the proteasome through an interaction between its UBL domain and the S2/PSMD2 proteasome subunit (4). The UBA domains of Rad23A bind mono- and polyubiquitin and are thought to shuttle proteins modified with Lys48-linked polyubiquitin chains to the proteasome for degradation (1,5-7). In addition to its role as an ubiquitin-binding protein, Rad23A also participates in nucleotide excision repair (NER) by binding and stabilizing the NER DNA binding protein XPC (2,8).
- Verma, R. et al. (2004) Cell 118, 99-110.
- Masutani, C. et al. (1994) EMBO J 13, 1831-43.
- Walters, K.J. et al. (2003) Proc Natl Acad Sci U S A 100, 12694-9.
- Elsasser, S. et al. (2002) Nat Cell Biol 4, 725-30.
- Raasi, S. et al. (2005) Nat Struct Mol Biol 12, 708-14.
- Nathan, J.A. et al. (2013) EMBO J 32, 552-65.
- Elsasser, S. et al. (2004) J Biol Chem 279, 26817-22.
- Ng, J.M. et al. (2003) Genes Dev 17, 1630-45.
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