Rad23B (D4W7F) Rabbit mAb #13525
- WB
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 53 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The UV excision repair protein Rad23B is a multi-domain scaffold protein that plays an important role in ubiquitin-dependent proteasomal degradation. Rad23B contains an amino-terminal ubiquitin-like (UbL) domain that facilitates interaction with the S5a/PSMD4 subunit of the proteasome 19S regulatory complex (2,3). In addition, Rad23B contains a central ubiquitin-associated domain (UBA1) and a carboxy-terminal UBA2 domain, which bind mono- and polyubiquitin with distinct specificities (4). Research studies demonstrate that Rad23B binds specifically to K48-ubiquitinated proteins to facilitate recruitment of target proteins to the proteasome (5). Between the paired UBA domains, Rad23B contains an XPC-binding domain that facilitates binding to XPC and recruitment to DNA lesions (6), as well as the binding of peptide:N-glycanase that is critical for recruitment of ubiquitinated ERAD substrates to the proteasome (7). Research studies have shown that targeted deletion of the murine Rad23b locus impairs embryonic development, suggesting that Rad23B is essential for mammalian development (8).
- Verma, R. et al. (2004) Cell 118, 99-110.
- Ryu, K.S. et al. (2003) J Biol Chem 278, 36621-7.
- Walters, K.J. et al. (2003) Proc Natl Acad Sci U S A 100, 12694-9.
- Raasi, S. et al. (2005) Nat Struct Mol Biol 12, 708-14.
- Nathan, J.A. et al. (2013) EMBO J 32, 552-65.
- Masutani, C. et al. (1994) EMBO J 13, 1831-43.
- Lee, J.H. et al. (2005) Proc Natl Acad Sci U S A 102, 9144-9.
- Ng, J.M. et al. (2002) Mol Cell Biol 22, 1233-45.
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