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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Rad52 Antibody #3425

Filter:
  • WB
  • IF
Western Blotting Image 1: Rad52 Antibody
Western blot analysis of extracts from K562 cells, using Rad52 Antibody.
This product is discontinued

Inquiry Info. # 3425

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Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 40
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Rad52 Antibody detects endogenous levels of total Rad52 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Hamster

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human Rad52. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Repair of DNA double-stranded breaks (DSBs) can occur via nonhomologous end joining (NHEJ), homologous recombination (HR), or other DSB repair pathways (1). In yeast, one family of proteins involved in HR is the Rad52 group, which includes Rad52 itself, Rad51, and Rad54 (1). Vertebrate cells lacking Rad51, Rad52, or Rad54 often show enhanced sensitivity to ionizing radiation (IR) or chromosomal abnormatlities (2-4). In response to IR-induced DNA damage, ATM activates the kinase c-Abl, which phosphorylates Rad51 at Tyr54 and Tyr315, and Rad52 at Tyr104, causing translocation of these proteins to sites of DNA strand breaks/subnuclear foci (5-7).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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