Render Target: STATIC
Render Timestamp: 2025-01-02T11:49:02.516Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-08-01 15:23:26.007
Product last modified at: 2025-01-01T09:04:10.616Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Ran Antibody #4462

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Hm Mk B
    SENSITIVITY Endogenous
    MW (kDa) 24
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Ran Antibody detects endogenous levels of total Ran protein. The antibody does not cross-react with other small GTPases of the Ras family.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Bovine

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    D. melanogaster, Xenopus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3).
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