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Product last modified at: 2025-01-01T09:04:43.149Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RanGAP1 (D2T7T) Rabbit mAb #36067

Filter:
  • WB
  • IP
  • eCLIP
Western Blotting Image 1: RanGAP1 (D2T7T) Rabbit mAb
Western blot analysis of extracts from various cell lines using RanGAP1 (D2T7T) Rabbit mAb.

To Purchase # 36067

Cat. # Size Qty. Price Ships
36067T 20 µl
$153
36067S 100 µl
$339

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 68, 82
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • eCLIP-eCLIP 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
eCLIP 1:200
For more information about the RBP-eCLIP service please visit Eclipsebio.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

RanGAP1 (D2T7T) Rabbit mAb recognizes endogenous levels of total RanGAP1 protein. This antibody detects unmodified and SUMO-1 modified RanGAP1 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RanGAP1 protein.

Background

Ran GTPase activating protein 1 (RanGAP1) regulates GTP hydrolysis by Ran, which is required for the ability of Ran to regulate nucleocytoplasmic shuttling (1). A significant fraction of cellular RanGAP1 is covalently modified by SUMO-1, which is required for relocalization of RanGAP1from the cytoplasm to the nuclear pore complex and the mitotic spindle (2-5). Research studies demonstrate that RanGAP1 sumoylation is required for stable association of RanGAP1 with RanBP2. Together with the SUMO-conjugating enzyme UBC9/UBE2I, RanGAP1 and RanBP2 are part of a SUMO E3 ligase complex that is implicated in regulating nucleocytoplasmic protein trafficking (6-8). Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity (9,10).
For Research Use Only. Not For Use In Diagnostic Procedures.
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