RANK Ligand (L300) Antibody #4816
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 35-45 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
RANKL (1), also named TNF-related activation-induced cytokine (TRANCE) (2,9), osteoprotegerin ligand (OPGL) (3), osteoclast differentiation factor (ODF) (4), and TNFSF11, is a type II transmembrane protein of the TNF family that exists as both a membrane-bound and soluble form. It is an essential regulator of immune function and bone development and homeostasis (7,10,11). RANKL is predominately expressed in activated T cells, as well as the thymus, lymph node, and bone marrow and promotes dendritic cell survival. Deletion of RANKL in mice leads to severe osteoporosis with a loss of osteoclasts, defects in T and B cell differentiation, loss of lymph node development, and mammary gland development during pregnancy (12-14).
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- Wong, B.R. et al. (1997) J. Exp. Med. 186, 2075-80.
- Lacey, D.L. et al. (1998) Cell 93, 165-76.
- Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 3597-602.
- Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-5.
- Wong, B.R. et al. (1999) Mol. Cell 4, 1041-9.
- Walsh, M.C. and Choi, Y. Cytokine Growth Factor Rev. 14, 251-63.
- Nakashima, T. et al. (2003) Curr. Opin. Rheumatol. 15, 280-7.
- Wong, B.R. et al. (1997) J Biol Chem 272, 25190-4.
- Hofbauer, L.C. (1999) Eur J Endocrinol 141, 195-210.
- Theill, L.E. et al. (2002) Annu Rev Immunol 20, 795-823.
- Mizuno, A. et al. (1998) Biochem Biophys Res Commun 247, 610-5.
- Kong, Y.Y. et al. (1999) Nature 397, 315-23.
- Fata, J.E. et al. (2000) Cell 103, 41-50.
Limited Uses
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