Render Target: STATIC
Render Timestamp: 2025-01-22T02:14:18.887Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:57:42.903
Product last modified at: 2025-01-01T09:03:21.702Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RBM15 (E8Y8A) Rabbit mAb #60386

Filter:
  • WB
  • IP
  • eCLIP
Western Blotting Image 1: RBM15 (E8Y8A) Rabbit mAb
Western blot analysis of extracts from various cell lines using RBM15 (E8Y8A) Rabbit mAb.

To Purchase # 60386

Cat. # Size Qty. Price Ships
60386T 20 µl
$153
60386S 100 µl
$339

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 110
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • eCLIP-eCLIP 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
eCLIP 1:200
For more information about the RBP-eCLIP service please visit Eclipsebio.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

RBM15 (E8Y8A) Rabbit mAb recognizes endogenous levels of total RBM15 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala557 of human RBM15 protein.

Background

RBM15 is an RNA-binding protein that is part of the WTAP-METTL3 m6A methyltransferase complex. RBM15 and related RBM15B interact with WTAP to recruit the complex to target mRNAs, and are critical to XIST-mediated gene silencing (1). RBM15 can recruit splicing factors such as SF3B1 to mRNA to promote alternative splicing. Expression levels of RBM15 can be regulated by PRMT1, which can methylate R578, resulting in RBM15 ubiquitinylation and degradation (2). This process is critical in acute megakaryoblastic leukemia, a cancer type where RBM15 is fused to the MKL-1 gene and PRMT1 is overexpressed (3). RBM15 normally plays roles in hematopoietic development and myeloid differentiation, where it can regulate the levels of c-Myc (4-6).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.