Render Target: STATIC
Render Timestamp: 2024-09-30T10:49:46.371Z
Commit: 53da7bbf777da8a463c431a64421017667e9ff1d
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. RBM8A (E5Z8T) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RBM8A (E5Z8T) Rabbit mAb #5386

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 21
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RBM8A (E5Z8T) Rabbit mAb recognizes endogenous levels of total RBM8A protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val70 of human RBM8A protein.

    Background

    The exon junction complex (EJC) plays a critical role in splicing, translation, mRNA localization, as well as nonsense-mediated decay. It comprises three core proteins, eIF4A3, Mago homolog (MAGOH), and RBM8A, as well as the peripheral factor MLN51 (1-4). The EJC proteins act as a binding platform for auxiliary factors that control mRNA export and nonsense-mediated decay (NMD) (5). RBM8A and MAGOH bind to key NMD factor UPF3B and dock around 20 nucleosides upstream of exon-exon junctions, which helps facilitate proper splicing and NMD functions (6-8). During translation, ribosomes will determine whether NMD factors are needed when encountering a stop codon. If EJCs exist downstream of the stop codon, the ribosome will recruit the SURF complex, and UPF1 phosphorylation will help facilitate the decay of prematurely terminated mRNAs (9,10). The EJC also is involved in the cell cycle as eIF4A3 is phosphorylated at threonine 163 by CDK1 and CDK2, which inhibits NMD function and prevents complex formation (11,12). Aberrant RBM8A expression causes cell cycle dysfunction, while low MAGOH expression affects neurological development and proliferation (13,14). EJC members have been implicated in a wide array of diseases and cancer, including hepatocellular carcinoma, glioblastoma, and neurological disorders (15-17).
    1. Schlautmann, L.P. and Gehring, N.H. (2020) Biomolecules 10, 866. doi: 10.3390/biom10060866.
    2. Leung, C.S. and Johnson, T.L. (2018) Mol Cell 72, 799-801.
    3. Boehm, V. and Gehring, N.H. (2016) Trends Genet 32, 724-735.
    4. Le Hir, H. and Séraphin, B. (2008) Cell 133, 213-6.
    5. Le Hir, H. et al. (2001) EMBO J 20, 4987-97.
    6. Kim, V.N. et al. (2001) Science 293, 1832-6.
    7. Gehring, N.H. et al. (2003) Mol Cell 11, 939-49.
    8. Fribourg, S. et al. (2003) Nat Struct Biol 10, 433-9.
    9. Isken, O. and Maquat, L.E. (2008) Nat Rev Genet 9, 699-712.
    10. Kashima, I. et al. (2006) Genes Dev 20, 355-67.
    11. Mazloomian, A. et al. (2019) Commun Biol 2, 165.
    12. Ryu, I. et al. (2019) Cell Rep 26, 2126-2139.e9.
    13. Ishigaki, Y. et al. (2013) Exp Biol Med (Maywood) 238, 889-97.
    14. Silver, D.L. et al. (2013) Dev Biol 375, 172-81.
    15. Liang, R. et al. (2017) Oncol Rep 37, 2167-2176.
    16. Lin, Y. et al. (2021) Front Oncol 11, 736941.
    17. Brunetti-Pierri, N. et al. (2008) Nat Genet 40, 1466-71.

    Database Links

    UniProt ID: Q9Y5S9


    Entrez-Gene Id: 9939


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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