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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RBM9 (E7W6U) Rabbit mAb #37315

Filter:
  • WB
  • IP
Western Blotting Image 1: RBM9 (E7W6U) Rabbit mAb
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or RBM9 siRNA (+), using RBM9 (E7W6U) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 37315

Cat. # Size Qty. Price
37315T 20 µl
$168
37315S 100 µl
$371

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 50, 60
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

RBM9 (E7W6U) Rabbit mAb recognizes endogenous levels of total RBM9 protein. This antibody detects multiple isoforms of RBM9.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala77 of human RBM9 protein.

Background

RBM9, also known as RBFOX2, is an RNA-binding protein involved in the regulation of tissue specific alternative splicing (1). RBM9 is widely expressed in many tissues throughout various developmental stages. Several isoforms of this protein are generated by variable use of multiple alternatively spliced internal exons and alternative splice sites (2-4). RBM9 is predominantly localized in the nucleus, where it regulates alternative pre-mRNA splicing. This factor was also identified to be a novel constituent of cytoplasmic stress granules (SGs), and its RNA binding activity is necessary for its localization into SGs (5). Notably, RBM9 dissociation from SGs suppresses cancer progression (6). The splicing-regulatory role of RBM9 has also been implicated as a tumor suppressor in pancreatic ductal adenocarcinoma (PDA) and could serve as a therapeutic approach for metastatic PDA (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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