RBMX/hnRNP G (D7C2V) Rabbit mAb #14794
- WB
- IP
Supporting Data
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 42 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly265 of human RBMX/hnRNP G protein.
Background
The X-linked RNA binding motif protein (RBMX, hnRNP G) is a multi-functional protein that is part of a heterogeneous nuclear ribonucleoprotein complex (1,2). This widely expressed protein is involved in the control of pre-mRNA splicing as part of the spliceosome. RBMX is important for the alternative splicing of many pre-mRNAs, including those that encode for dystrophin, tropomyosin, and survival motor neuron protein (SMN) in skeletal muscle and cardiac muscle (3,4). The RBMX protein is essential for the maintenance of proper sister chromatid cohesion prior to sister chromosome segregation during mitosis (5). Research studies show that RBMX accumulates at sites of DNA damage and that the presence of RBMX is required for homologous recombination repair (6).
- Heinrich, B. et al. (2009) J Biol Chem 284, 14303-15.
- Martinez-Contreras, R. et al. (2007) Adv Exp Med Biol 623, 123-47.
- Nasim, M.T. et al. (2003) Hum Mol Genet 12, 1337-48.
- Hofmann, Y. and Wirth, B. (2002) Hum Mol Genet 11, 2037-49.
- Matsunaga, S. et al. (2012) Cell Rep 1, 299-308.
- Adamson, B. et al. (2012) Nat Cell Biol 14, 318-28.
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