RHBDF2 (E7U2K) Rabbit mAb #26182

Filter:

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	97
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

RHBDF2 (E7U2K) Rabbit mAb recognizes endogenous levels of total RHBDF2 protein. Bands of unknown identity are detected at 50 kDa and 160 kDa.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys36 of human RHBDF2 protein.

Background

Inactive rhomboid factor 2 (RHBDF2), also known as RHBDL6 or iRhom2, is a proteolytically inactive member of the transmembrane family of rhomboid serine protease family (1). Inactive rhomboid family members have been implicated in several physiological and pathophysiological conditions, including wound healing, cancer, neurodegenerative diseases, and inflammatory disorders (reviewed in 2). RHBDF2 facilitates endoplasmic reticulum trafficking of the metalloprotease ADAM17 (also known as TACE) to the membrane, thereby promoting EGF family growth factor and cytokine shedding and signaling (1). Loss of RHBDF2 results in significantly reduced secretion of ligand-mediated secretion of these factors. Inherited dominant mutations in the RHBDF2 gene are the genetic basis for the cancer-susceptibility syndrome tylosis with esophageal cancer (TOC) (3). Elevated expression of RHBDF2 in oral squamous carcinoma is associated with tumor progression and poor patient prognosis (4). The E3 ubiquitin ligase TRIM31 directly targets RHBDF2 and facilitates its proteasomal degradation (5). Hepatocyte specific ablation of TRIM31 facilitates phenotypes of non-alcoholic fatty liver disease (5).

Database Links

UniProt ID: Q6PJF5

Entrez-Gene Id: 79651

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.