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Render Timestamp: 2024-12-27T11:22:56.081Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-18 18:01:09.215
Product last modified at: 2024-12-21T00:00:08.790Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Rho-GTPase Antibody Sampler Kit

Rho-GTPase Antibody Sampler Kit #9968

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Rho-GTPase Antibody Sampler Kit contains reagents to examine aspects of cell migration, adhesion, proliferation and differentiation in cells. This kit includes enough primary and secondary antibodies to perform two Western blot experiments per each primary antibody.

    Specificity / Sensitivity

    Cdc42 (11A11) Rabbit mAb detects endogenous levels of total Cdc42 protein and does not cross-react with other small GTPases. Phospho-Rac1/cdc42 (Ser71) Antibody detects endogenous Rac1/cdc42 only when phosphorylated at Ser71 and may also recognize phospho-RhoA (Ser73). Rac 1/2/3 Antibody detects endogenous levels of total Rac1/2/3 proteins. RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. RhoB Antibody recongnizes endogenous levels of RhoB. RhoC (D40E4) XP® Rabbit mAb recongnizes endogenous levels of RhoC.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr138 of human Rac1, corresponding to residues surrounding Ser71 of human Rac1/cdc42, and corresponding to residues near the carboxy terminus of RhoB . Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
    Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Lys135 of human Cdc42, residues near the carboxy terminus of human RhoA, and to residues corresponding to the carboxy terminus of human RhoC.

    Background

    The Rho family of small GTPases, including Rho, Rac, and cdc42, act as molecular switches to regulate processes such as cell migration, adhesion, proliferation and differentiation (1). RhoA, RhoB and RhoC are all highly homologous but appear to have divergent biological functions. The best characterized of these proteins, RhoA, regulates acomysin contractility, cytokinesis, focal adhesion assembly and cell polarity (2-5). Mammalian Rac exists as three isoforms (Rac1, Rac2 and Rac3) that show high sequence similarity. Well-characterized Rac1 and cdc42 are ubiquitously expressed and play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, and cell growth and development (6). Phosphorylation of Rac1 at a putative Akt site (Ser71) may limit Rac1 activity through inhibition of GTP binding (7). Rac2 is expressed in cells of hematopoietic origin, while Rac3 is highly expressed in brain and in many other tissues. The Vav family of guanine-nucleotide exchange factors mediates activation of Rho/Rac family small GTPases (8). Negative regulation of Rho-activity members of the p190 RhoGAP family (p190-A and p190-B) may be controlled by Src phosphorylation of Tyr residues, activating the p190 GAP domain (8-10). Furthermore, Rho GDP dissociation inhibitor (RhoGDI) associates with Rho/Rac to negatively regulate nucleotide exchange membrane localization (11).

    Database Links

    UniProt ID: P08134 , P15153 , P63000 , P60763 , P62745 , P60953 , P61586


    Entrez-Gene Id: 389 , 5880 , 5879 , 5881 , 388 , 998 , 387


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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