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RhoA (67B9) Rabbit Monoclonal Antibody #2117

Filter:
  • WB
  • IHC
  • IF
Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb.

To Purchase # 2117

Cat. # Size Qty. Price
2117T 20 µl
$145
2117S 100 µl
$339

Product Specifications

REACTIVITY H M R Mk B
SENSITIVITY Endogenous
MW (kDa) 21
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • B-Bovine 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:250
Immunohistochemistry (Paraffin) 1:800
Immunofluorescence (Immunocytochemistry) 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #60382.

Protocol

Specificity / Sensitivity

RhoA (67B9) Rabbit Monoclonal Antibody recognizes endogenous levels of total RhoA protein. This antibody does not recognize the related proteins RhoB or RhoC.

Species Reactivity:

Human, Mouse, Rat, Monkey, Bovine

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human RhoA.

Background

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).

Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility (4), cytokinesis (5), focal adhesion assembly (6) and cell polarity (7).

Alternate Names

Aplysia ras-related homolog 12; ARH12; ARHA; EDFAOB; epididymis secretory sperm binding protein; h12; oncogene RHO H12; ras homolog family member A; ras homolog gene family, member A; Rho cDNA clone 12; RHO12; RHOA; RHOH12; small GTP binding protein RhoA; Transforming protein RhoA

Pathways

Explore pathways related to this product.


For Research Use Only. Not for Use in Diagnostic Procedures.
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