RIP3 (D8J3L) Rabbit mAb (BSA and Azide Free) #92131
- ELISA
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46-62 |
| Source/Isotype | Rabbit IgG |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #15828
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Receptor-interacting protein 3 (RIP3) was originally found to interact with RIP and the TNF receptor complex to induce apoptosis and activation of NF-κB (9,10). It has subsequently been shown that the association between RIP and RIP3 is a key component of a signaling pathway that results in programmed necrosis (necroptosis), a necrotic-like cell death induced by TNF in the presence of caspase inhibitors (11-13). RIP3 is phosphorylated at Ser227 and targets the phosphorylation of mixed lineage kinase domain-like protein (MLKL), which is critical for necroptosis (14).
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- Ting, A.T. et al. (1996) EMBO J 15, 6189-96.
- Kelliher, M.A. et al. (1998) Immunity 8, 297-303.
- Devin, A. et al. (2000) Immunity 12, 419-29.
- Zhang, S.Q. et al. (2000) Immunity 12, 301-11.
- Lin, Y. et al. (1999) Genes Dev 13, 2514-26.
- Yu, P.W. et al. (1999) Curr Biol 9, 539-42.
- Sun, X. et al. (1999) J Biol Chem 274, 16871-5.
- Zhang, D.W. et al. (2009) Science 325, 332-6.
- He, S. et al. (2009) Cell 137, 1100-11.
- Cho, Y.S. et al. (2009) Cell 137, 1112-23.
- Sun, L. et al. (2012) Cell 148, 213-27.
Limited Uses
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