Render Target: STATIC
Render Timestamp: 2024-10-30T09:42:48.326Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-08-01 15:28:29.066
Product last modified at: 2024-05-30T07:02:31.469Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

RNF128 Antibody #71590

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60-80
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RNF128 Antibody recognizes endogenous levels of total RNF128 protein. This antibody detects a 40 kDa band of unknown origin in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RNF128 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    RNF128, also known as gene related to anergy in lymphocytes (GRAIL), is a RING finger protein family E3 ubiquitin ligase that participates in T cell anergy induction (1,2). RNF128 is a type I transmembrane protein, made up of a zinc RING finger, coiled-coil, and protease-associated domain (1,2). Expression of RNF128/GRAIL has been shown to inhibit cytokine transcription and CD4 T cell proliferation, as well as regulate naïve T cell tolerance induction and Treg cell function (2-4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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