Render Target: STATIC
Render Timestamp: 2024-12-20T11:20:03.051Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:28:44.698
Product last modified at: 2024-11-08T23:30:10.610Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

RPRD1B Antibody #74693

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 37
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    RPRD1B Antibody recognizes endogenous levels of total RPRD1B protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RPRD1B protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Regulation of nuclear pre-mRNA domain-containing 1B (RPRD1B), also known as cell cycle-related and expression-elevated protein in tumor (CREPT), is a transcriptional regulator that interacts with the POLR2A catalytic subunit of RNA polymerase II (Pol II) (1). It is also an oncoprotein that promotes cell proliferation and is upregulated in a variety of cancers, including gastric, colorectal, and non-small cell lung cancer (NSCLC) (2-4). RPRD1B contains a CTD-interacting domain (CID), which interacts with the C-terminal domain (CTD) of Pol II to regulate transcription termination (5). It is believed that this association enhances the binding of Pol II to the CCND1 promoter, thereby increasing cyclin D1 transcription and promoting tumorigenesis (6). Specifically, RPRD1B promotes chromatin looping of the CCND1 gene to recycle Pol II from the termination site to the promoter region (6). RPRD1B may also regulate the transcription of various genes through associations with STAT3 and the histone acetyltransferase p300 (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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