Render Target: STATIC
Render Timestamp: 2024-12-26T11:54:56.423Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-18 16:03:17.073
Product last modified at: 2024-12-19T12:45:09.962Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SAFB1 (F5Z6A) Rabbit mAb #64996

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 160
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SAFB1 (F5Z6A) Rabbit mAb recognizes endogenous levels of total SAFB1 protein. This antibody does not cross-react with SAFB2 protein. This antibody detects a 75 kDa band of unknown origin in mouse and rat. Reactivity by immunofluorescence is human only.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala909 of human SAFB1 protein.

    Background

    Scaffold attachment factor B1 and B2 (SAFB1/B2) are homologs that bind to scaffold or matrix attachment region DNA elements (S/MAR DNA) (1,2). SAFB1 and SAFB2 bind to estrogen receptor to inhibit its activity, an action enhanced by tamoxifen treatment and opposed by histone deacetylase activity (2-4). SAFB1 and SAFB2 contain an RNA recognition motif and can interact with various hnRNP complexes and serine/arginine-rich splicing factors to influence pre-mRNA splicing (5-7). SAFB1 is also capable of helping in the DNA damage response by allowing yH2AX spreading (8). Knockdown of SAFB1 results in increased SAFB2 expression, and knockdown of SAFB2 results in increased SAFB1 expression (9). Despite their high homology, SAFB1 and SAFB2 have some non-redundant functions. SAFB1 knockout mice have a high degree of growth and fertility issues, while SAFB2 knockout mice have a normal phenotype but aberrations in Sertoli cells (10). SAFB proteins are expressed lowly in breast cancers with poor prognosis (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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