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SARS-CoV-2 Envelope Protein Antibody

SARS-CoV-2 Envelope Protein Antibody #74698

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Supporting Data

REACTIVITY	Vir
SENSITIVITY	Endogenous
MW (kDa)	12
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

Vir-Virus

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SARS-CoV-2 Envelope Protein Antibody recognizes endogenous levels of total SARS-CoV-2 envelope protein in SARS-CoV-2-infected cells.

Species Reactivity:

Virus

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of SARS-CoV-2 envelope protein.

Background

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Envelope protein is transcribed and translated from nested subgenomic RNA of the SARS-CoV-2 genome. Despite the SARS-CoV-2 envelope protein being very small, it possesses a number of functionalities that are critical for viral assembly and release. It is also a major virulence factor, a viroporin that contributes to the host immune mediated cytokine storm (3). A C-terminal motif on envelope protein binds to and mislocates a number of cell tight junction proteins in lung epithelial cells which may contribute to alveolar damage and acute respiratory distress syndrome (ARDS) (4).

Database Links

UniProt ID: P0DTC4

Entrez-Gene Id: 43740570

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For Research Use Only. Not For Use In Diagnostic Procedures.

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