SARS-CoV-2 Envelope Protein Antibody #74698
- WB
Supporting Data
| REACTIVITY | Vir |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
SARS-CoV-2 Envelope Protein Antibody recognizes endogenous levels of total SARS-CoV-2 envelope protein in SARS-CoV-2-infected cells.
Species Reactivity:
Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of SARS-CoV-2 envelope protein.
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Envelope protein is transcribed and translated from nested subgenomic RNA of the SARS-CoV-2 genome. Despite the SARS-CoV-2 envelope protein being very small, it possesses a number of functionalities that are critical for viral assembly and release. It is also a major virulence factor, a viroporin that contributes to the host immune mediated cytokine storm (3). A C-terminal motif on envelope protein binds to and mislocates a number of cell tight junction proteins in lung epithelial cells which may contribute to alveolar damage and acute respiratory distress syndrome (ARDS) (4).
Limited Uses
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