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Product last modified at: 2025-01-01T09:00:37.566Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SARS-CoV-2 Nsp9 (E3E3X) Rabbit mAb #95561

Filter:
  • WB
Western Blotting Image 1: SARS-CoV-2 Nsp9 (E3E3X) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or transiently transfected with plasmid encoding Myc/DDK-tagged SARS-CoV-2 Nsp9 protein (lane 2), mock-infected Vero-E6 cells (lane 3) or SARS-CoV-2-infected Vero-E6 cells (lane 4), using SARS-CoV-2 Nsp9 (E3E3X) RAbbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). SARS-CoV-2-infected Vero-E6 cells courtesy of Dr. Mohsan Saeed, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.

To Purchase # 95561

Cat. # Size Qty. Price Ships
95561T 20 µl
$153
95561S 100 µl
$339

Supporting Data

REACTIVITY Vir
SENSITIVITY Endogenous
MW (kDa) 10
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • Vir-Virus 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SARS-CoV-2 Nsp9 (E3E3X) Rabbit mAb recognizes endogenous levels of total SARS-CoV-2 Nsp9 protein in infected cells.

Species Reactivity:

Virus

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly93 of SARS-CoV-2 Nsp9 protein.

Background

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2).

Nsp9 is a non-structural protein that is derived from ORF1a and ORF1b (2). Although the function of Nsp9 is not completely understood, it is known to be an RNA-binding protein with a unique architecture that interacts with the viral polymerase holoenzyme complex. It is well conserved between coronaviruses and is thought to be essential for viral replication, thus making it a target of therapeutic development (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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