SARS-CoV-2 Spike Protein (E2M5O) Mouse mAb (BSA and Azide Free) #10947
Supporting Data
| REACTIVITY | Vir |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Usage Information
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The SARS-CoV-2 spike protein contains a novel tetrabasic "furin cleavage site" (FCS) at the S1/S2 junction. Research studies suggest this site is cleaved by proprotein convertases (e.g., furin) or lysosomal proteases (e.g., cathepsin L) (5,6). S1/S2 cleavage elicits a confirmational change in the spike protein that positions elements of the trimeric RBD in an exposed "up" position, priming it for interaction with host receptor proteins. Cleavage can occur at multiple steps of the viral lifecycle, including during viral packaging, or upon contact of the intact virion with the host cell surface. This novel cleavage event has been suggested to contribute to the high infectivity rate of the SARS-CoV-2 virus (7).
The SARS-CoV-2 virus has been shown to utilize the angiotensin-converting enzyme 2 (ACE2) protein as its primary receptor for cellular entry (8). However, research studies have suggested that other cell surface proteins may serve as receptors or co-receptors for SARS-CoV-2. These include neuropilin-1 (NPN1), a single-pass transmembrane receptor that can function as part of a semaphorin receptor complex, and as a vascular endothelial growth factor (VEGF) receptor (9), and Basigin/EMMPRIN (CD147), a type I integral membrane receptor belonging to the immunoglobulin superfamily (10).
- Zhou, P. et al. (2020) Nature 579, 270-273.
- Tortorici, M.A. and Veesler, D. (2019) Adv Virus Res 105, 93-116.
- Li, F. et al. (2006) J Virol 80, 6794-800.
- Li, F. (2016) Annu Rev Virol 3, 237-261.
- Coutard, B. et al. (2020) Antiviral Res 176, 104742.
- Jaimes, J.A. et al. (2020) iScience 23, 101212.
- Hasan, A. et al. (2021) J Biomol Struct Dyn 39, 3025-3033.
- Shang, J. et al. (2020) Nature 581, 221-224.
- Cantuti-Castelvetri, L. et al. (2020) Science 370, 856-860.
- Wang, K. et al. (2020) Signal Transduct Target Ther 5, 283.
Limited Uses
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