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Render Timestamp: 2024-11-22T11:43:08.684Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:47.896
Product last modified at: 2024-11-11T13:15:58.839Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. SARS-CoV-2 Spike Protein (S1-NTD) (E7M5X) Mouse mAb

SARS-CoV-2 Spike Protein (S1-NTD) (E7M5X) Mouse mAb #42172

Filter:
  • WB
  • F

    Supporting Data

    REACTIVITY Vir
    SENSITIVITY Endogenous
    MW (kDa) 110, 220
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Flow Cytometry (Live) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-2 Spike Protein (S1-NTD) (E7M5X) Mouse mAb recognizes endogenous levels of total SARS-CoV-2 spike protein. This antibody detects full-length protein, and also detects the S1 fragment generated by furin cleavage. It does not cross-react with spike proteins from SARS or MERS coronaviruses.

    Species Reactivity:

    Virus

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of SARS-CoV-2 spike protein.

    Background

    The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2’ sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9).

    Database Links

    UniProt ID: P0DTC2


    Entrez-Gene Id: 43740568


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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