Render Target: STATIC
Render Timestamp: 2025-01-08T11:48:03.266Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-09-30 01:56:07.630
Product last modified at: 2025-01-01T09:05:09.865Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SAT1 (D1T7M) Rabbit mAb #61586

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 18
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SAT1 (D1T7M) Rabbit mAb recognizes endogenous levels of total SAT1 protein.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp132 of human SAT1 protein.

    Background

    Spermidine/spermine N1-acetyltransferase 1 (SAT1) is a key enzyme in polyamine metabolism. It acts by acetylating spermidine and spermine using acetyl-coenzyme A (CoA), which alters their charge and facilitates their secretion (1,2). SAT1 activity is tightly controlled in cells, but increases quickly by excess polyamines. Its activity can also be induced by a number of other stimuli, such as oxidative stress, heat shock, insulin-like growth factor-I, and cytotoxins (2-4). Research studies have found that SAT1 is overexpressed in glioblastoma multiforme (GBM), which suggest that SAT1 may be a potential therapeutic target for GBM (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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