Render Target: STATIC
Render Timestamp: 2024-12-20T11:45:31.760Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:44.608
Product last modified at: 2024-12-12T17:45:13.635Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SCARB1 (E9H4F) Rabbit mAb #90332

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:150 - 1:600
    Immunofluorescence (Frozen) 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SCARB1 (E9H4F) Rabbit mAb recognizes endogenous levels of total SCARB1 protein. Non-specific staining was observed in normal human small intestine by immunohistochemistry.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile487 of mouse SCARB1 protein.

    Background

    Scavenger receptor class B member 1 (SCARB1, SR-B1) is a multi-ligand cell-surface receptor that facilitates uptake of lipids from cholesterol into cells (1,2). SCARB1 is part of the CD36 scavenger receptor superfamily, which is composed of many proteins with varied structures, expressions, and functions across tissue and cell types (1-3). SCARB1 recognizes a variety of ligands: high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS) (2,4,5). SCARB1 knockout mice have elevated HDL levels and increased susceptibility to atherosclerosis when given a high-fat diet, indicating its importance in the regulation of cholesterol homeostasis (6). Additionally, SCARB1 has a role in antigen cross-presentation from bacteria and viruses (1-3). SCARB1 has been reported to be an entry factor for the Hepatitis C virus (7). In endothelial cells and macrophages, SCARB1 has an anti-inflammatory and pro-survival role, which provides protection against atherosclerosis and helps to clear apoptotic cells (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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