SDF1/CXCL12 (D8G6H) Rabbit mAb #97958
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10 |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #89194.
Protocol
Specificity / Sensitivity
SDF1/CXCL12 (D8G6H) Rabbit mAb recognizes endogenous levels of total SDF1/CXCL12 protein by immunohistochemistry on paraffin-embedded tissue sections. This antibody recognizes both SDF1/CXCL12α and SDF1/CXCL12β.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SDF1/CXCL12 protein.
Background
The stromal cell derived factor 1 (SDF1/CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1/CXCL12 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1/CXCL12 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1/CXCL12 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1/CXCL12 isoforms (SDF1/CXCL12α and SDF1/CXCL12β) that have different properties and biological activities (7). Additional isoforms of SDF1/CXCL12 have been reported (8,9).
- Maekawa, T. and Ishii, T. (2000) Intern Med 39, 90-100.
- Ray, N. and Doms, R.W. (2006) Curr Top Microbiol Immunol 303, 97-120.
- Sugiyama, T. et al. (2006) Immunity 25, 977-88.
- Ara, T. et al. (2003) Immunity 19, 257-67.
- Kucia, M. et al. (2004) J Mol Histol 35, 233-45.
- Ratajczak, M.Z. et al. (2006) Leukemia 20, 1915-24.
- De La Luz Sierra, M. et al. (2004) Blood 103, 2452-9.
- Gleichmann, M. et al. (2000) Eur J Neurosci 12, 1857-66.
- Yu, L. et al. (2006) Gene 374, 174-9.
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