Render Target: STATIC
Render Timestamp: 2024-12-20T11:02:15.065Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-17 23:02:07.515
Product last modified at: 2024-12-18T16:30:09.399Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SERPINB3 (F2I8O) Rabbit mAb #39027

Filter:
  • WB
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SERPINB3 (F2I8O) Rabbit mAb recognizes endogenous levels of total SERPINB3 protein. This antibody detects a 60 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu323 of human SERPINB3 protein.

    Background

    Serpin family B member 3 (SERPINB3), also known as squamous cell carcinoma antigen 1 (SCCA1), belongs to the serpin family and inhibits papain-like cysteine proteases (1). SERPINB3 is unique to the other serpins, as it lacks inhibitory activity against serine proteases but has inhibitory activity against papain-like cysteine proteases cathepsins K, L, and S (1,2). It may function as an inhibitor to modulate the host immune response against tumor cells and can act as a UV-induced apoptosis inhibitor by suppressing the activity of c-Jun NH2-terminal kinase 1 (JNK1) (1,3). SERPINB3 is expressed in normal squamous epithelium and in several organs, including tongue, esophageal, uterine, cervical, and vaginal epithelium. It is also expressed in Hassall’s corpuscles of the thymus and some areas of the skin (4). SERPINB3 has been found to be overexpressed and implicated in several types of cancer, including hepatocellular carcinoma (HCC), lung, ovarian, and breast cancer (5-8). It is also overexpressed and implicated in hepatoblastoma, autoimmune disorders, lung fibrosis, cholesteatoma, and skin disease (9-13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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