SERPINB9 (E9X9Z) Rabbit mAb (BSA and Azide Free) #88078

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	42
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

This product is the carrier free version of product #58551. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #58551

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

SERPINB9 (E9X9Z) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total SERPINB9 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human SERPINB9 recombinant protein and reacts with an epitope surrounding Met280.

Background

SERPINB9 (Sb9, PI-9) is a member of the serpin superfamily of proteins which bind to and inactivate serine proteinases (1,2). SERPINB9 is present in human CD4⁺ T cells, CD8⁺ T cells, NK cells, B cells, and myeloid cells, and is a physiological inhibitor of Granzyme B (GrB) (1,3,4). Granzyme B triggers apoptosis when released by cytotoxic T lymphocytes (1-4). SERPINB9 may function to protect effector and bystander cells from Granzyme B during an immune response (4). In mice, SPI6 is thought to be the functional homolog of SERPINB9 due to its inhibition of murine Granzyme B (2,5). SERPINB9 was recently identified as a potential immunotherapy target, due to its role in tumor cell survival through evasion of apoptosis (6).

Database Links

UniProt ID: P50453

Entrez-Gene Id: 5272

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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