Render Target: STATIC
Render Timestamp: 2024-11-21T13:51:10.375Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:56:07.739
Product last modified at: 2024-11-15T19:45:08.148Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SET1A (D3V9S) Rabbit mAb #61702

Filter:
  • WB
  • IP
  • ChIP
  • C&R
  • C&T

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 300
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    • C&R-CUT & RUN 
    • C&T-CUT & Tag 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Chromatin IP 1:100
    Chromatin IP-seq 1:100
    CUT&RUN 1:100
    CUT&Tag 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SET1A (D3V9S) Rabbit mAb recognizes endogenous levels of total SET1A protein. This antibody does not cross-react with other SET1/COMPASS proteins, including SET1B, MLL1, MLL2, MLL3, and MLL4.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro383 of human SET1A protein.

    Background

    The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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