Render Target: STATIC
Render Timestamp: 2025-01-03T11:51:33.335Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-11-13 16:01:06.467
Product last modified at: 2025-01-01T09:02:41.424Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. SFPQ (E9A7B) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SFPQ (E9A7B) Rabbit mAb #71992

Filter:
  • WB
  • IP
  • eCLIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 65, 75, 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • eCLIP-eCLIP 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    eCLIP 1:200
    For more information about the RBP-eCLIP service please visit Eclipsebio.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SFPQ (E9A7B) Rabbit mAb recognizes endogenous levels of total SFPQ protein. This antibody may detect a non-specific band of unknown identity at 32 kDa.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SFPQ protein.

    Background

    Splicing factor proline-glutamine rich (SFPQ), also commonly referred to as PTB-associated splicing factor (PSF), is an essential and ubiquitous nucleic acid-binding protein that is involved in a wide range of cellular processes. SFPQ belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with paraspeckle protein 1 (PSPC1) and non-POU domain-containing octamer-binding protein (NONO) (1). DBHS proteins are a unique family of RNA-binding proteins that recruit various proteins and nucleic acids involved in transcriptional activation and repression, transcript localization, splicing, and DNA damage repair (2). SFPQ and NONO are also core components of paraspeckles, which are subnuclear bodies distinct from nuclear speckles that are defined by the colocalization of SFPQ, NONO, and other proteins with the long noncoding RNA NEAT1 (2). The biological function of paraspeckles is currently unknown, but they are believed to be important for gene regulation in the presence of cellular stressors (3). Although SFPQ is typically localized to the nucleus, multiple studies indicate that cytoplasmic SFPQ levels are important for proper motor neuron differentiation and maintenance (4,5). SFPQ has also been linked to multiple neurological disorders, including Alzheimer’s disease (AD) and amyotrophic lateral sclerosis (ALS) (6-9).

    Database Links

    UniProt ID: P23246


    Entrez-Gene Id: 6421


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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