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Render Timestamp: 2024-11-26T10:55:45.907Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-09-30 01:58:46.081
Product last modified at: 2024-11-06T13:30:16.688Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. SH2D1B/EAT-2 (E2P5P) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SH2D1B/EAT-2 (E2P5P) Rabbit mAb #58173

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 15
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SH2D1B/EAT-2 (E2P5P) Rabbit mAb recognizes endogenous levels of total SH2D1B/EAT-2 protein. This antibody does not cross-react with SH2D1A/SAP protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly92 of human SH2D1B/EAT-2 protein.

    Background

    SH2 domain-containing protein 1B/EWS/FLI1-activated transcript 2 (SH2D1B/EAT-2) belongs to the SAP family of cytoplasmic signaling adaptor proteins. Structurally, SH2D1B/EAT-2 is highlighted by a single SH2 domain and a short C-terminus containing multiple tyrosine residues whose phosphorylation regulate SH2D1B/EAT-2 function (1,2). Research studies have demonstrated that SH2D1B/EAT-2 is recruited to multiple SLAM family receptors in a phosphotyrosine-dependent manner and that phosphorylation of its C-terminal tyrosine residues is required for control of downstream signaling (3-5). SH2D1B/EAT-2 expression has been detected in multiple hematopoietic cell lineages, including NK cells and macrophages (4,6). Indeed, in murine NK cells, tyrosine phosphorylation of SH2D1B/EAT-2 mediates inhibitory signaling downstream of 2B4/SLAMF4 to suppress effector functions (4).

    Database Links

    UniProt ID: O14796


    Entrez-Gene Id: 117157


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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