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Product last modified at: 2025-01-01T09:06:05.550Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

SHANK2 Antibody #12218

Filter:
  • WB
  • IP
Western Blotting Image 1: SHANK2 Antibody
Western blot analysis of extracts from the indicated rat brain synaptic fractions using SHANK2 Antibody. Separation of the different synaptic fractions was confirmed using PSD95 (D27E11) XP® Rabbit mAb #3450 and Syntaxin 6 (C34B2) Rabbit mAb #2869. Equal loading of each fraction was assessed using β-Tubulin (9F3) Rabbit mAb #2128. Fractionation of the different synaptic compartments was carried out as described by Phillips, G.R. et al. (2001) Neuron 32, 63-77. PAZ, Pre-synaptic active zone.

To Purchase # 12218

Cat. # Size Qty. Price Ships
12218S 100 µl
$306

Supporting Data

REACTIVITY M R
SENSITIVITY Endogenous
MW (kDa) 165
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein.

Species Reactivity:

Mouse, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Human, Monkey, Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val537 of human SHANK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
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