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SHMT2 (E7F4Q) Rabbit mAb #33443

Filter:
  • WB
  • IP
  • IHC
Western blot analysis of extracts from RH-30, MDA-MB-231, and HeLa cells using SHMT2 (E7F4Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 33443

Cat. # Size Qty. Price
33443T 20 µl
$162
33443S 100 µl
$357

Product Specifications

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 52
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SHMT2 (E7F4Q) Rabbit mAb recognizes endogenous levels of total SHMT2 protein. This antibody does not cross-react with SHMT1 protein. Species cross-reactivity for IHC-P is human only.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly451 of human SHMT2 protein.

Background

Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5,10-methylene-THF (1,2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).

Alternate Names

1-C; 1C; GLY A+; GLYA; glycine auxotroph A, human complement for hamster; Glycine hydroxymethyltransferase; GLYM; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); Serine hydroxymethyltransferase, mitochondrial; Serine methylase; SHMT; SHMT2; threonine aldolase

For Research Use Only. Not for Use in Diagnostic Procedures.
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