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SHMT2 (E7X5B) Rabbit mAb #93566

Filter:
  • F
Flow Cytometry Image 1: SHMT2 (E7X5B) Rabbit mAb
Flow cytometric analysis of HeLa cells (blue) and RH30 cells (green) using SHMT2 (E7X5B) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

To Purchase # 93566

Cat. # Size Qty. Price
93566T 20 µl
$153
93566S 100 µl
$339

Product Specifications

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 52
Source/Isotype Rabbit IgG
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SHMT2 (E7X5B) Rabbit mAb recognizes endogenous levels of total SHMT2 protein. This antibody does not cross-react with SHMT1 protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu402 of human SHMT2 protein.

Background

Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxymethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5,10-methylene-THF (1,2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).

Alternate Names

1-C; 1C; GLY A+; GLYA; glycine auxotroph A human complement for hamster; Glycine hydroxymethyltransferase; GLYM; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 mitochondrial; Serine hydroxymethyltransferase mitochondrial; Serine methylase; SHMT; SHMT2; threonine aldolase

For Research Use Only. Not for Use in Diagnostic Procedures.
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