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Product last modified at: 2025-01-01T09:04:15.707Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SIGIRR (E5M2V) Rabbit mAb #32850

Filter:
  • WB
  • IP
Western Blotting Image 1: SIGIRR (E5M2V) Rabbit mAb
Western blot analysis of extracts from various cell lines using SIGIRR (E5M2V) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 32850

Cat. # Size Qty. Price Ships
32850T 20 µl
$153
32850S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 75
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SIGIRR (E5M2V) Rabbit mAb recognizes endogenous levels of total SIGIRR protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly391 of human SIGIRR protein.

Background

SIGIRR (Single Ig IL-1-related receptor), also known as IL-1R8, belongs to the IL-1 receptor family characterized by the presence of a common intracellular Toll-IL-1 resistance (TIR) domain and extracellular Ig-like domains (1-3). SIGIRR is widely expressed in several epithelial tissues such as liver, lung, and kidney, and in leukocytes including monocytes, T and B lymphocytes, dendritic cells, and natural killer (NK) cells. It negatively modulates the activation upon ligand binding of certain TIR-containing receptors including members of IL-1 receptor family and TLR family (4-6). Recently SIGIRR was identified as a crucial checkpoint receptor in NK cells and a potential therapeutic target in several pathological conditions (7,8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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