Render Target: STATIC
Render Timestamp: 2024-12-26T11:59:51.054Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-26 21:24:06.635
Product last modified at: 2024-12-17T18:54:14.049Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Siglec-10 (D4E3Z) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Siglec-10 (D4E3Z) Rabbit mAb #41262

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 92, 100-105
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Siglec-10 (D4E3Z) Rabbit mAb recognizes endogenous levels of total Siglec-10 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro250 of mouse Siglec-10 protein.

    Background

    Siglec-10 is a sialic acid-binding Ig-like lectin that has been shown to negatively regulate innate and adaptive immune system function (1). Siglec-10 is a type 1 transmembrane protein that has four extracellular Ig-like domains, a transmembrane region, and a cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (2). Siglec-10 is expressed in dendritic cells (DCs), monocytes, B cells, NK cells, and T cells (2,3). Siglec-10 interacts with CD24 to suppress immune responses to danger associated molecular patterns (DAMPs) by associating with the tyrosine phosphatase SHP-1, a negative regulator of nuclear factor-kappa B (NF-κB) (4). Siglec-10 has been shown to bind soluble CD52 leading to the impairment of phosphorylation of the T cell receptor–associated kinases Lck and Zap70 and T cell activation (3). It has been proposed that this mechanism of suppression could be involved in T cell homeostasis and the prevention of type I diabetes (3). Siglec-10 has also been identified as the leukocyte ligand for vascular adhesion protein-1 (VAP-1), which plays a key role in leukocyte trafficking (5). Lectin galactoside-binding soluble 3 binding protein (LGALS3BP), a tumor-associated immunomodulatory ligand, has also been shown to bind Siglec-10 (6).

    Database Links

    UniProt ID: Q80ZE3


    Entrez-Gene Id: 243958


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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