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SirT4 Antibody

SirT4 Antibody #69786

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Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	29-33
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SirT4 Antibody recognizes endogenous levels of total SirT4 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly235 of human SirT4 protein. Antibodies are purified by peptide affinity chromatography.

Background

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, initially classified as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT4, a mammalian homolog of Sir2, is localized to the mitochondria and has been implicated in the regulation of cell metabolism, oxidative stress, genomic stability, and aging (2,3). In addition to deacetylase activity, multiple additional enzymatic activities have been attributed to SirT4, including protein deacylation, ADP-ribosylation, and NAD-dependent protein lipoamidase activity. SirT4 regulates the metabolism of glutamine, lipids, and glucose through direct deacetylation of malonyl CoA decarboxylase (MCD) (4), ADP-ribosylation of glutamine dehydrogenase (5), and hydrolysis of the lipoamide cofactors from the E2 component dihydrolipoyllysine acetyltransferase (DLAT) of the pyruvate dehydrogenase complex (PDHC) (6). SirT4 is a tumor suppressor protein, and expression is reduced or lost in a large number of cancers, resulting in the acceleration of tumor morbidity and metastasis (2,3).

Database Links

UniProt ID: Q9Y6E7

Entrez-Gene Id: 23409

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For Research Use Only. Not For Use In Diagnostic Procedures.

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