Sirtuin Antibody Sampler Kit #9787
Product Information
Kit Usage Information
Protocols
- 2314: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow
- 5360: Western Blotting, Immunoprecipitation (Agarose)
- 5490: Western Blotting, Immunoprecipitation (Magnetic)
- 7074: Western Blotting
- 8782: Western Blotting
- 9475: Western Blotting
- 12486: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence
- 12650: Western Blotting
Product Description
Specificity / Sensitivity
Source / Purification
Background
SirT3 exists in human cells in two forms, including a full-length, nuclear (44 kDa) protein and a processed (28 kDa) protein found exclusively in the mitochondria (4-6). Full-length SirT3 protein is processed in the mitochondrial matrix by mitochondrial matrix processing peptidase (MMP) (5). Both full-length and processed SirT3 are active enzymes that deacetylate histone H3 at Lys9 and histone H4 at Lys16 in vitro (4). SirT3 also deacetylates Lys642 of acetyl-CoA synthetase 2 (AceCS2) and activates AceCS2 activity in the mitochondria (7).
SirT5 is localized to the mitochondria and has been implicated in the regulation of cell metabolism (8,9). Nuclear SirT6 is a chromatin-associated protein that promotes normal maintenance of genome integrity as mediated by the base excision repair (BER) pathway (10-12). Mammalian SirT7 is localized to the nucleolus and is prominently expressed in hematopoietic cells, especially myeloid progenitor cells (13). SirT7 is recruited to chromatin by sequence-specific DNA binding transcription factors such as Elk-4, where it facilitates transcriptional repression through deacetylation of histone H3 at Lys18 (14).
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- North, B.J. et al. (2003) Mol Cell 11, 437-44.
- Vaquero, A. et al. (2006) Genes Dev 20, 1256-61.
- Scher, M.B. et al. (2007) Genes Dev 21, 920-8.
- Schwer, B. et al. (2002) J Cell Biol 158, 647-57.
- Onyango, P. et al. (2002) Proc Natl Acad Sci U S A 99, 13653-8.
- Schwer, B. et al. (2006) Proc Natl Acad Sci U S A 103, 10224-9.
- Newman, J.C. et al. (2012) J Biol Chem 287, 42436-43.
- He, W. et al. (2012) Trends Endocrinol Metab 23, 467-76.
- Mostoslavsky, R. et al. (2006) Cell 124, 315-29.
- Liszt, G. et al. (2005) J Biol Chem 280, 21313-20.
- Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35.
- Voelter-Mahlknecht, S. et al. (2006) Int J Oncol 28, 899-908.
- Barber, M.F. et al. (2012) Nature 487, 114-8.
Limited Uses
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