SIX1 (D5S2S) Rabbit mAb #16960
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 36 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #73583.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
In contrast to the Drosophila ortholog, the vertebrate SIX1 gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13).
- Kumar, J.P. (2009) Cell Mol Life Sci 66, 565-83.
- Fischbach, K.F. and Technau, G. (1984) Dev Biol 104, 219-39.
- Fischbach, K.F. and Heisenberg, M. (1981) Proc Natl Acad Sci U S A 78, 1105-9.
- Boucher, C.A. et al. (1996) Genomics 33, 140-2.
- Pignoni, F. et al. (1997) Cell 91, 881-91.
- Ford, H.L. et al. (1998) Proc Natl Acad Sci U S A 95, 12608-13.
- Coletta, R.D. et al. (2004) Proc Natl Acad Sci U S A 101, 6478-83.
- Oliver, G. et al. (1995) Development 121, 4045-55.
- Kaiser, R. et al. (2007) Am J Med Genet A 143A, 2185-8.
- Fujimoto, Y. et al. (2013) Dev Cell 26, 416-30.
- Liu, Y. et al. (2013) PLoS One 8, e67762.
- Behbakht, K. et al. (2007) Cancer Res 67, 3036-42.
- Ng, K.T. et al. (2006) Br J Cancer 95, 1050-5.
Limited Uses
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