Render Target: STATIC
Render Timestamp: 2024-11-15T10:17:51.930Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:59:40.727
Product last modified at: 2024-11-11T12:30:09.315Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SLC25A1 (E7B2B) Rabbit mAb #97835

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:300 - 1:1200
    Immunofluorescence (Frozen) 1:50
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SLC25A1 (E7B2B) Rabbit mAb recognizes endogenous levels of total SLC25A1 protein. Species cross-reactivity for IHC-P is human only.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the mitochondrial matrix-facing domains of human SLC25A1 protein. The antigen has been further characterized as corresponding to residues surrounding Arg173 of human SLC25A1 protein.

    Background

    Members of the solute carrier 25 (SLC25) family transport molecules over the mitochondrial inner membrane (1,2). Mutations in these carriers often result in rare but severe pathologies (3). SLC25A1 (tricarboxylate transport protein, CIC, or citrate transport protein) transports citrate from the inner mitochondrial matrix to the cytosol in exchange for malate. This function makes it a key source of cytoplasmic acetyl-CoA and downstream lipid biosynthesis (4). Mutations in SLC25A1 result in D-2- and L-2-hydroxyglutaric aciduria, causing lowered levels of citrate and isocitrate in urine, with elevated urinary levels of other TCA-cycle intermediates (3). Cancer cells have elevated mitochondrial membrane potential and require SLC25A1 to maintain and promote tumorigenesis (5,6). SLC25A1 is a transcriptional target of p53, and inhibiting SLC25A1 activity can blunt mutant p53-driven tumor growth (6). Inhibitors of SLC25A1 have been found to slow the growth of pancreatic and non-small cell lung cancers (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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