Render Target: STATIC
Render Timestamp: 2024-11-22T10:45:57.900Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:54:51.016
Product last modified at: 2024-09-30T08:01:35.426Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Smac/Diablo (79-1-83) Mouse mAb #2954

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 21
    Source/Isotype Mouse IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Smac/Diablo (79-1-83) Mouse mAb detects endogenous levels of processed and unprocessed human Smac/Diablo. The antibody reacts weakly with mouse and rat Smac/Diablo.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a full length peptide corresponding to human Smac/Diablo raised in E-coli.

    Background

    Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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