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Render Timestamp: 2024-12-26T11:31:05.284Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:11.887
Product last modified at: 2024-12-17T18:46:50.368Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. SMARCC1/BAF155 (D7F8S) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SMARCC1/BAF155 (D7F8S) Rabbit mAb #11956

Filter:
  • WB
  • IP
  • ChIP
  • C&R
  • C&T

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 155
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    • C&R-CUT & RUN 
    • C&T-CUT & Tag 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:100
    Chromatin IP-seq 1:100
    CUT&RUN 1:100
    CUT&Tag 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SMARCC1/BAF155 (D7F8S) Rabbit mAb recognizes endogenous levels of total SMARCC1/BAF155 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly975 of human SMARCC1/BAF155 protein.

    Background

    ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

    SMARCC1/BAF155 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by BRG1 in vitro (10). SMARCC1 is an essential part of the mouse embryonic stem cell specific SWI/SNF complex (esBAF), which is necessary for early embryogenesis, especially proper brain and visceral endoderm development (11-13).

    Database Links

    UniProt ID: Q92922


    Entrez-Gene Id: 6599


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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