SMARCD1/BAF60A (E7W9W) Rabbit mAb #35070
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
SMARCD/BAF60 proteins are BAF family members that, along with SMARCC1/BAF155A, serve as an initial subcomplex critical for the formation of the intact BAF complex (9). These proteins serve as an interface between the BAF complex and many other key transcription factors, including glucocorticoid receptor and p53 (10,11). SMARCD1/BAF60A has been implicated in stem cell differentiation by controlling H3K27me3 distribution (12). Furthermore, SMARCD1/BAF60A has been shown to play a role in Notch signaling in glioblastomas (13).
- Trotter, K.W. and Archer, T.K. (2008) Nucl Recept Signal 6, e004.
- Trotter, K.W. and Archer, T.K. (2007) Mol Cell Endocrinol 265-266, 162-7.
- Sif, S. et al. (2001) Genes Dev 15, 603-18.
- Zhang, H.S. et al. (2000) Cell 101, 79-89.
- Underhill, C. et al. (2000) J Biol Chem 275, 40463-70.
- Magnani, L. and Cabot, R.A. (2009) Reproduction 137, 23-33.
- Medina, P.P. et al. (2008) Epigenetics 3, 64-8.
- Medina, P.P. et al. (2008) Hum Mutat 29, 617-22.
- Mashtalir, N. et al. (2018) Cell 175, 1272-1288.e20.
- Hsiao, P.W. et al. (2003) Mol Cell Biol 23, 6210-20.
- Oh, J. et al. (2008) J Biol Chem 283, 11924-34.
- Alajem, A. et al. (2015) Cell Rep 10, 2019-31.
- Zhu, Y. et al. (2021) Mol Neurobiol 58, 1438-1452.
Limited Uses
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