SMARCE1/BAF57 (E6H5J) Rabbit mAb #33360
- WB
- IP
- ChIP
- C&R
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 57 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:50 |
| CUT&RUN | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
SMARCE1/BAF57 is a core component of the SWI/SNF complex and directly binds other transcription factors and co-factors, including androgen receptor and estrogen receptor. Binding of SMARCE1/BAF57 can directly affect the transcriptional activity of nuclear receptors, influencing the expression of target genes (9-12). Mutations of SMARCE1/BAF57 have been described in Coffin-Siris syndrome (13). SMARCE1/BAF57 has also been shown to contribute to invasion of some early-stage breast lesions by forming a complex with ILF3, an event independent of the SWI/SNF complex (14).
- Trotter, K.W. and Archer, T.K. (2008) Nucl Recept Signal 6, e004.
- Trotter, K.W. and Archer, T.K. (2007) Mol Cell Endocrinol 265-266, 162-7.
- Sif, S. et al. (2001) Genes Dev 15, 603-18.
- Zhang, H.S. et al. (2000) Cell 101, 79-89.
- Underhill, C. et al. (2000) J Biol Chem 275, 40463-70.
- Magnani, L. and Cabot, R.A. (2009) Reproduction 137, 23-33.
- Medina, P.P. et al. (2008) Epigenetics 3, 64-8.
- Medina, P.P. et al. (2008) Hum Mutat 29, 617-22.
- Link, K.A. et al. (2005) Mol Cell Biol 25, 2200-15.
- Belandia, B. et al. (2002) EMBO J 21, 4094-103.
- García-Pedrero, J.M. et al. (2006) J Biol Chem 281, 22656-64.
- Link, K.A. et al. (2008) Cancer Res 68, 4551-8.
- Tsurusaki, Y. et al. (2012) Nat Genet 44, 376-8.
- Sokol, E.S. et al. (2017) Proc Natl Acad Sci U S A 114, 4153-4158.
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