Render Target: STATIC
Render Timestamp:
4/4/2025, 6:25:56 AM EDT
4/4/2025, 10:25:56 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-04-01 22:06:28.081
Product last modified at: 2025-04-03T08:00:12.321Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SMG6 (F6A1U) Rabbit mAb #35537

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SMG6 (F6A1U) Rabbit mAb recognizes endogenous levels of total SMG6 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala125 of human SMG6 protein.

    Background

    Nonsense-mediated decay (NMD) is a process by which prematurely terminated transcripts are degraded before they can be translated (1-3). There are two differential processes for NMD in higher eukaryotes, endonucleolytic and exonucleolytic, which are governed by SMG6 (also known as EST1A) or SMG5 and SMG7, respectively (1,4-8). SMG5-7 are structurally similar, but SMG6 contains a PIN domain with endonucleolytic activity. While SMG5 contains a similar domain, it lacks critical residues for endonuclease activity, and SMG7 does not contain a PIN domain (7). All three proteins are recruited to UPF1 when it becomes phosphorylated by SMG1, and from there, the NMD process significantly diverges. Upon binding to UPF1, SMG5 and SMG7 can recruit decaying and deadenylating protein complexes, resulting in subsequent mRNA degradation via the XRN1 exonuclease and the exosome (8,9). SMG6, however, promotes decay through cleaving nonspecific sites within 40 nucleotides of the premature termination codon (PTC) (7,10). Interestingly, SMG6-mediated decay depends on either a functional SMG5 or SMG7 (11). NMD pathways are species-specific, with Drosophila relying more on SMG6-mediated decay and yeast utilizing the decaying and deadenylating approach to mRNA turnover (12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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