SMN1 (2F1) Mouse mAb #12976
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
Protocol
Specificity / Sensitivity
SMN1 (2F1) Mouse mAb recognizes endogenous levels of total SMN1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human SMN1 protein.
Background
Survival of Motor Neuron 1 (SMN1) is essential for the maturation of small nuclear ribonucleoproteins (snRNPs) (1,2). SMN1 plays a role in the assembly of spliceosomal snRNPs in the cytoplasm, together with the Gemin proteins, and may also participate in the transport of snRNPs into the nucleus (3-6). SMN1 also participates in the maturation and turnover of snRNPs in nuclear foci Gemini bodies (gems) (7). In addition to the maturation of spliceosomal snRNPs, SMN1 has also been proposed to directly regulate pre-mRNA splicing (8). Researchers have found mutations and deletions of the SMN1 gene are found in 95% of Spinal Muscular Atrophy (SMA) neuromuscular disorder cases (1,9).
Limited Uses
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