SMRT (D8D2L) Rabbit mAb #62370

Filter:

WB
ChIP

Supporting Data

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	270
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
ChIP-Chromatin Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Chromatin IP	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SMRT (D8D2L) Rabbit mAb recognizes endogenous levels of total SMRT protein. This antibody does not cross-react with NCoR1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to residues near the amino terminus of human SMRT protein.

Background

The most well characterized nuclear receptor corepressors are NCoR1 (nuclear receptor corepressor) and its close paralog NCoR2, also know as SMRT (silencing mediator for retinoic acid and thyroid hormone receptors) (1,2). NCoR1 and SMRT function to transcriptionally silence various unliganded, DNA bound non-steroidal nuclear receptors by serving as a large molecular scaffold that bridges the receptors with multiple chromatin remodeling factors that repress nuclear receptor-mediated gene transcription, in part, through deacetylation of core histones surrounding target promoters. Indeed, the N-terminal portion of NCoR1 and SMRT possess multiple distinct transcriptional repression domains (RDs) responsible for the recruitment of additional components of the corepressor complex such as HDACs, mSin3, GPS2, and TBL1/TBLR1. In between the RDs lies a pair of potent repressor motifs known as SANT motifs (SWI3, ADA2, N-CoR, and TFIIIB), which recruit HDAC3 and histones to the repressor complex in order to enhance HDAC3 activity (3). The C-terminal portion of NCoR1 and SMRT contain multiple nuclear receptor interaction domains (NDs), each of which contains a conserved CoRNR box (or L/I-X-X-I/V-I) motif that allow for binding to various unliganded nuclear hormone receptors such as thyroid hormone (THR) and retinoic acid (RAR) receptors (4,5).

Database Links

UniProt ID: Q9Y618

Entrez-Gene Id: 9612

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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